Strains were isolated from tocantins savannah soils. In alkaline soils, phosphate can precipitate to form calcium phosphates, including rock phosphate fluorapatite and francolite, which are insoluble in soil. Pikovskayas agar was modified by sundara rao and sinha 2 for detection of phosphatesolubilizing bacteria from soil. Phosphate solubilization and mobilization in soil through. Effects of phosphate solubilization and phytohormone production of. Effect of abiotic stress on phosphate solubilization by. The soluble phosphate concentration was quantified at. The soil samples were characterized physicochemically before planting. This study was undertaken to explore the role of trichoderma sp. The present study assessed phosphate solubilization by the fungus trichoderma as well as phytase enzyme activity and growth effects on seedlings of the mangrove avicennia marina. Phosphate solubilization by trichoderma koningiopsis nbripr5. The study explores high psolubilizing potential of trichoderma sp. Trichoderma species that solubilized phosphate and produced iaa, provided significant results in the accumulation of biomass of the crops, with relative efficiency of 276% for mint and 141% for basil, in relation to the control.
Phosphate solubilization by trichoderma cultures disappearance of tcp within 72 h indicates the high potential of trichoderma for solubilization of inorganic bound phosphate tcp, which might have been subsequently taken up by the fungus for cellular processes. Solubilisation of phosphate and micronutrients by trichoderma harzianum and its relationship with the promotion of tomato plant growth. Ten isolates of trichoderma derived from the rhizosphere of a. Pdf phosphate solubilization and phytohormone production. Phosphate solubilization potential and phosphatase activity of rhizospheric trichoderma spp. Solubilization of rock phosphate was indicated by an increase in the concentration of soluble ca fig. Antagonistic and phosphate solubilization potential of. Phosphate solubilization and growth promotion of rubber tree. Drt1 and prt1 showing maximum psolubilizing potential were selected for phosphatase enzyme induction studies using pikovskayas broth medium amended with three different combinations of bound phosphate tcp and available phosphates kh 2 po 4. Such solubilization and nutrient translocation by fungi may be environmentally significant since it may influence the location of fungal species and relocation of otherwise immobile elements such as metals and phosphate in the soil. Phosphate solubilization by trichoderma species is reported invitro as well as in rhizosphere.
In the liquid medium with calcium phosphate a greater amount of soluble p 71. Mechanisms of phosphate solubilization the principal mechanism for mineral phosphate solubilization is the production of organic acids, and acid phosphatases play a major role in the mineralization of organic phosphorus in soil. Evaluation of the phosphate solubilization potential of trichoderma. The soluble phosphate concentration was quantified at 3, 6, 9 and 12 days after transplanting. Phosphate solubilizing ability of the tested fungi was confirmed by the appearance of a clear halo zone around the colony development at 4 days of the incubation periods. Satya vani2 1department of environmental sciences, college of science and technology, andhra university, visakhapatnam, andhra pradesh, india530003. Acid phosphatases acpases are produced by a variety of fungi and have gained attention due their biotechnological potential in industrial, diagnosis and bioremediation processes.
Phosphate solubilization potential and phosphatase activity of. This activity may explain, at least partially, the ability of t22 to increase plant growth. These enzymes play a specific role in scavenging, mobilization and acquisition of phosphate, enhancing soil fertility and plant growth. The trichoderma isolates showed phosphate solubilization capacity and iaa synthesis. Evaluation of the phosphate solubilization potential of.
These results reveal the potential of trichoderma spp. Protease were released by trichoderma viride to play an active role in the biological control of some plant pathogens. Soil phosphates are rendered available either by plant roots or by soil microorganisms. Fungal discs from four day old potato dextrose agar cultures each of aspergillus, penicillium and trichoderma 4 mm. Microbial phosphorus solubilization and its potential for. Evaluation of phosphodissolvent iaa producing strains of. Phosphate solubilization potential and phosphatase activity. An overview krishnananda pralhad ingle1 and dipika ashokrao padole2 biotechnology centre, dr. In this study, trichoderma isolated from soils of the amazon rainforest demonstrated the potential for phosphate solubilization and increased soybean plant growth, highlighting the importance of. Pdf phosphate solubilization and phytohormone production by. Nov 30, 1998 data on solubilization of cuo and fe 2 o 3 in liquid culture were not conclusive because of highly variable data due to initial partial solubility of these minerals. Solubilization of metal oxides by trichoderma involves both chelation and reduction. Potential phosphate solubilization and aia production of trichoderma spp.
Dorcas zunigasilgado environmental engineering study program, faculty of architecture and engineering, mayor college of antioquia university institution, career. Effects of phosphate solubilization and phytohormone production of trichoderma asperellum q1 on promoting cucumber growth under salt stress. This is the first report of the ability of a trichoderma strain to solubilize insoluble or sparingly soluble minerals. College of life science, shandong normal university, jinan 250014, p. Therefore, phosphate dissolving soil organisms play a part in correcting phosphorus deficiency of crop plants 1. Fungal discs from four day old potato dextrose agar cultures each. Sep 01, 2010 determination of phosphatase enzyme activity.
Solubilization of phosphates and micronutrients by the. Prospects of using phosphate solubilizing pseudomonas as bio. It is generally accepted that the major mechanism of mineral phosphate solubilization is the action. Microbial phosphorus solubilization and its potential for use. Cicer arietinum, nutrient uptake, phosphate solubilization, plant yield, trichoderma spp. Evaluation of phosphodissolvent iaa producing strains of trichoderma, spp.
Prospects of using phosphate solubilizing pseudomonas as. Bacterial colonies forming halo zones were calcium phosphate. Maharashtra, india abstract the region where soil and roots makes contact is called rhizosphere. Twentyone isolates were evaluated in vitro for their ability to solubilize calcium phosphate and. It is possible that the isolates produced either the same blend of metabolites in diff erent proportions or completely diff erent chemical compounds 3. Phosphate solubilizer bacterial inoculants was spread on modified pikovskaya produces a clear zone around the bacterial colonies on agar plates for phosphate solubilization assay as media containing insoluble mineral phosphate such as tri described 8. Panjabrao deshmukh krishi vidyapeeth, akola, maharashtra, india corresponding author a b s t r a c t calcium phosphate, aluminum phosphate and ferrous phosphate and thus becomes unavailable to plants. Jadhav department of microbiology, shivneri mahavidyalaya, shirur a. This work aimed to isolate and evaluate the phosphate solubilization and phytohormone production abilities of endophytic and rhizosphere fungi belonging to the genus trichoderma isolated from guanandi calophyllum brasiliense cambess. Phosphate solubilization potential and phosphatase. The principal mechanism for solubilization of soil p is lowering of soil ph by microbial production of organic acids or the release of protons 3, 5, 6, 9, 10, 18, 23, 30. In this study, a new acpase from trichoderma harzianum, named acpase ii, was. Phosphorous and phosphate solubilising bacteria and their role in plant nutrition m.
Inorganic phosphate solubilization by phosphate solubilizing. Pdf solubilisation of phosphate and micronutrients by. We investigated the capability of the plantgrowthpromoting and biocontrol fungus trichoderma harzianum rifai 129522 t22 to solubilize in vitro some insoluble or sparingly soluble minerals via three possible mechanisms. To evaluate the phosphate solubilization capacity of the trichoderma spp. Trichoderma virens mediated rock phosphate solubilization.
Solubilization of phosphates and micronutrients by the plantgrowth. Jun 18, 2019 the present study was undertaken to estimate in vitro the capacity of twentythree isolates of trichoderma, isolated from soil and roots collected from adjacent sites to the khouribga and benguerir phosphate mines morocco, and seven isolates from the laboratorys mycoteca to solubilize the tricalcium phosphate. Pdf phosphate solubilization potential and phosphatase activity. Phosphate solubilization and growth promotion of rubber. The strains were isolated, molecularly identified and grown in vitro.
Azomonas agilis had potent phosphate solubilization and also strongly produce some organic acids. Twentyone isolates were evaluated in vitro for their ability to solubilize calcium phosphate and synthesize indole acetic acid iaa. The most common p minerals found in soils are presented in table 1 in order of decreasing solubility. Therefore, phosphatedissolving soil organisms play a part in correcting phosphorus deficiency of crop plants 1. Solubilization of phosphates and micronutrients by the plant. Solubilization of inorganic phosphate by rhizospheric fungi. Pikovskayas agar was modified by sundara rao and sinha 2 for detection of phosphate solubilizing bacteria from soil. In alkaline soils, phosphate can precipitate to form calcium phosphates, including rock phosphate fluorapatite and francolite, which are. The present study was undertaken to estimate in vitro the capacity of twentythree isolates of trichoderma, isolated from soil and roots collected from adjacent sites to the khouribga and benguerir phosphate mines morocco, and seven isolates from the laboratorys mycoteca to solubilize the tricalcium phosphate. Production of organic acids by trichoderma species is one of the mechanisms for phosphate solubilization. This study aims to evaluate the phosphate solubilization potential of the trichoderma spp. Similarly results was recorded in solubilization of phosphate in broth culture such as aspergillus niger 256 and 328. Based on the psolubilization studies, two isolates of trichoderma sp. Pdf this study aims to evaluate the phosphate solubilization potential of the trichoderma spp.
Solubilization of calcium phosphate as a consequence of. All these isolates showed, after three days of incubation, on nbrip medium. Tricalcium phosphate solubilizing abilities of trichoderma spp. Pdf phosphate solubilization potential and phosphatase. Effect of trichoderma on soil phosphate solubilization and.
As microorganisms are efficient solubilizers of rock phosphate, attempts were made to employ a local isolate of trichodrema virens, which has a proven track record for erp solubilization, to increase the solubility of erp with the aim of developing an environmentally friendly liquid p fertilizer. The fungal isolates were identified as aspergillus chevalieri, fusarium moniliforme, and trichoderma harzianum. Phosphorous and phosphate solubilising bacteria and their. P sol b bacillus megaterium biofertilizer approved for use.
Tricalcium phosphate solubilizing abilities of trichoderma. Trichoderma harzianum produces a new thermally stable acid. Plant growth promoting activities of fungi and their. Phosphate solubilization and mobilization in soil through microorganisms under arid ecosystems 95 phosphate. The mechanism of mineral phosphate solubilization by phospho bacteria strains has been associated with the release of low molecular weight organic acids as described by by halder et al in 1990, goldstein in 1995 and kim et al in 1997 in different experiments. Phosphate solubilization potential and phosphatase activity of rhizospheric trichoderma spp anil kapri. Three zincphosphatesolubilizing fungi zpsf were isolated from rhizospheric soil cultivated with sorghum bicolor l. Pdf evaluation of the phosphate solubilization potential of. Quantitative estimation of phosphate solubilization the pikovskaya broth was amended with 5 g l1 each of tricalcium phosphate 17% p and ferric phosphate 18% p separately in two different set. Phosphate solubilization ability of nbripr5 was optimum over a ph range of 79 and nearly 50% ability was retained between ph 9 and 10. All 9 trichoderma isolates were found to solubilize insoluble tricalcium phosphate to various extents. Rice was inoculated with the trichoderma strains in greenhouse.
Phosphate solubilization by trichoderma koningiopsis nbri. Rice was inoculated with the trichoderma strains in greenhouse conditions with 10 to. Solubilization of inorganic phosphate by rhizospheric. T22 was able to solubilize mno 2, metallic zinc, and rock phosphate. The aim of this study was to determine the efficiency of trichoderma spp. Solubilization involves a number of intermediary states that can be studied by a variety of. Strengite and variscite are too insoluble to contribute much to plant nutrition. The objective of this study was to investigate the contribution of sqrt037 to p and microelement fe, mn, cu and zn nutrition in tomato plants grown in soil and in hydroponic conditions. However, the level of phosphorus in the culture filtrate. Trichoderma harzianum strain sqrt037 is a biocontrol agent that has been shown to enhance the uptake of nutrients macro and microelements by plants in fields. Effects of phosphate solubilization and phytohormone. The consequence of this phenomenon was the solubilization of calcium phosphate.
Solubilisation of phosphate and micronutrients by trichoderma. In vitro selection of phosphate solubilizing strains of trichoderma spp. Phosphate solubility and biocontrol activity of trichoderma harzianum 594 antibiotics that have diff erent eff ects on diff erent test fungi. Antagonistic and phosphate solubilization potential of trichoderma sp from rhizosphere of red gram cultivated in marathwada region r. The results showed that halo zone formation by zpsf on pikovskaya pvk agar medium plates was an indicator of zinc phosphate zp solubilization. In vitro selection of phosphate solubilizing strains of. Phosphate solubilization by trichoderma viride in cadmium stress and at a temperature range of 045 c has been earlier reported rawat and tewari, 2011.
1571 826 750 705 1127 16 1464 508 16 1305 712 1550 382 1589 35 462 1053 313 1563 871 388 1399 1036 816 64 1363 260 1387 911 450 164 943 430 976